Tolerance of rhizobia from chickpea nodules (Cicer arietinum L.) against abiotic factors
DOI:
https://doi.org/10.5281/zenodo.8321988Keywords:
factors abiotic, rhizobia, toleranceAbstract
Context: The stress abiotic alters the physiologic and metabolic processes of the plants, for what is considered the central cause of the losses of the cultivations, of there the necessity to study the effect of microorganisms that you/they contribute to counteract these effects.
Objective: To select for their tolerance in front of factors isolated abiotic of rhizobia coming from nodules of the cultivation of the chickpea (Cicer arietinum L.) to contribute to the adaptability of the cultivation before the same ones.
Methods: The tolerance was studied in front of isolated factors abiotic of eleven of rhizobia from chickpea nodules (Cicer arietinum L.). it was characterized under conditions in vitro its tolerance in front of different pH levels, chloride of sodium (NaCl), temperatures and drought.
Results: Eleven rhizobias was studied by its capacity to tolerate different pH levels, all grew in a range from 6 to 8. Of them, 4 isolated 8,5 grew to pH and to pH 9 were only able to grow two of them. To concentrations of NaCl, all grew to concentrations between 0,5 and 0,6%, starting from 0,7% NaCl, 7 of the isolated, alone only grew two of them they grew to concentrations between 1 and 1,5% NaCl and none tolerated 2% of NaCl. With respect to the tolerance to the different ranges of temperature, all grew to 35 oC, of them 63% grew to 36 oC and only two isolated they grew to 37 oC. Alone an isolated one tolerated between 38 and 39 oC and to 40 oC none showed growth. The tolerance a conditions of drought, only two isolated grew in presence de 3% PEG 6000.
Conclusions: Coming from nodules of the cultivation of the chickpea (Cicer arietinum L.) some rizobios was evaluated with tolerance in front of different pH levels, chloride of sodium (NaCl), temperatures and drought, what will allow contributing to the adaptability of the cultivation before these effects under the conditions of Cuba.
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